Ca²⁺ concentrations and transient numbers in acinar cells of both strains under agonist stimulation.

<p><i>A, B</i>. Maximum values of intracellular free Ca²⁺ concentration ([Ca²⁺]_i) [Ca²⁺]_i (<i>C_M</i>) in wild-type (WT; black filled symbols) and knockout (KO; red open symbols) acinar cells. Responses were induced by 10-min applications of various concentrations of cholecystokinin (CCK; A) or acetylcholine (ACh; B). The values in (A) and (B) were obtained from the same samples as those in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0037048#pone-0037048-g003" target="_blank">Fig. 3</a> (A) and <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0037048#pone-0037048-g003" target="_blank">Fig. 3</a> (B), respectively. Data are means ± SE of values obtained from the following numbers of cells: <b>in A</b>, 24 (1 pM), 23 (2 pM), 13 (10 pM), 21 (100 pM), 23 (500 pM), 18 (1 nM), or 21 (10 nM) different WT cells, and 17 (2 pM), 24 (10 pM), 12 (100 pM), 19 (500 pM), 14 (1 nM), or 21 (10 nM) different KO cells; <b>in B</b>, 26 (5 nM), 28 (10 nM), 27 (20 nM), 15 (50 nM), 17 (100 nM), 20 (500 nM), 17 (1 µM), 22 (10 µM), or 17 (100 µM) different WT cells, and 14 (10 nM), 19 (20 nM), 22 (50 nM), 28 (100 nM), 22 (500 nM), 31 (1 µM), 21 (10 µM), or 17 (100 µM) different KO cells. <i>C, D</i>. Average numbers of Ca²⁺ transients per minute in WT (black filled symbols) and KO (red open symbols) acinar cells. Responses were induced by 10-min applications of various concentrations of CCK (C) or ACh (D). Data are means ± SE of values obtained from the following numbers of cells: <b>in C</b>, 4 (1 pM), 6 (2 pM), 13 (10 pM), 21 (100 pM), 21 (500 pM), 18 (1 nM), or 18 (10 nM) different WT cells, and 1 (2 pM), 24 (10 pM), 12 (100 pM), 18 (500 pM), 14 (1 nM), or 14 (10 nM) different KO cells; <b>in D</b>, 4 (5 nM), 6 (10 nM), 10 (20 nM), 12 (50 nM), 9 (100 nM), 15 (500 nM), 7 (1 µM), 22 (10 µM), or 17 (100 µM) different WT cells, and 2 (20 nM), 6 (50 nM), 13 (100 nM), 8 (500 nM), 18 (1 µM), 18 (10 µM), or 17 (100 µM) different KO cells. Indexes * and ** indicate <i>p</i><0.05 and <i>p</i><0.01, respectively.