Activated Ly6G⁺ granulocytes protect from mortality during virus infection.

<p>(A) Cytokine gene expression in Ly6G⁺ enriched bone marrow cells. (B) Uptake of fluorescent latex beads by neutrophils (PI⁻CD45⁺Ly6G⁺CD11b⁺) in the lung of GCSF^+/+ and G-CSF^−/− mice four days after infection with SeV (n = 4). (C) Representative flow cytometry analysis of neutrophils (CD11b⁺Ly6c^int) and monocytes (CD11b⁺Ly6c^hi) in the blood of mice injected with a single dose of anti-Ly6G antibody (1A8) or an isotype control. Neutrophil depleted WT C57BL6 mice were infected with SeV and monitored for (D) weight loss (<i>p</i>>0.9 two way ANOVA for infected groups) and (E) survival (n = 10 for isotype treated, SeV infected and n = 20 for Ly6G depleted and SeV infected. Logrank test, <i>p</i><0.0001). Experiments shown are representative of two or three independent experiments.