Glycine-induced Ca²⁺ signalling in fura-2 loaded NPCs differentiated for 1 or 3 weeks.

<p>A, Changes in [Ca²⁺]_i were evoked by application of 100 µM glycine (Gly), 100 µM D-serine (D-Ser), 20 µM strychnine (Stry), and 50 mM KCl in cells differentiated for 3 weeks. The trace is the mean response of eight cells from a representative experiment. B, Summary of the [Ca²⁺]_i response amplitudes (n = 22–27 cells; means ± SEM) and fractions of cells (n = 60–75 from 86 cells) responding to different stimuli was obtained from 4 experiments as shown in A. C, NPCs differentiated for 1 week showed smaller increases in [Ca²⁺]_i upon application of 100 µM glycine and 50 mM KCl. The trace is the mean response of 8 cells from a representative experiment. D, Fractions of cells differentiated for 1 week (n = 7–29 of 139 cells) or 3 weeks (n = 60–75 from 86 cells) responding to different stimuli were obtained from 5 and 4 experiments as shown in A and C, respectively.