Genetic variants among the cisplatin-sensitive and -resistant ovarian cancer cells.

<p>(<b>A</b>) Western blotting was performed for the expression variations of prosurvival Bcl-2, Bcl-x_L and Mcl-1 and proapoptotic Bak and Bax proteins among the cisplatin-sensitive (A2780s, IGROV1 and OAW42) and -resistant (A2780cp, OVCAR-3 and SKOV3) ovarian cancer cells. β-actin was used as a loading control. (<b>B</b>) A2780s (p53 WT) and SKOV3 (p53 -/-) cells were treated with cisplatin (5 µg/mL) for 24 hours and analyzed for the expression of p53, p73, p21^waf1/cip1, NOXA and Bax by Western blotting. β-actin was used as a loading control. (<b>C</b>) OVCAR3 (harboring mutant p53 R248Q) and A2780cp (containing p53 wild-type gene sequence but showing loss of p53 function) cells were treated with cisplatin (5 µg/mL) for 24 hours and analyzed for the expression of p53, p73, p21^waf1/cip1, NOXA and Bax by Western blotting. β-actin was used as a loading control.