Pore-forming activity of DipA.

<p>(A) Single-channel conductance observed for DipA in a diphytanoyl phosphatidylcholine/<i>n</i>-decane (PC) membrane. About 10 ng ml⁻¹ of purified DipA of about was added to a PC lipid bilayer, bathed in 1 M KCl. (B) Histogram of individual single-channel events observed for purified DipA. The average single-channel conductance was 50 pS for a total number of 140 single steps; temperature = 20°C; voltage = 20 mV.