Detection of primary infected cells in dispersed TGEs at 2 dpi.

a<p>TGEs were infected either in the axonal or ganglion compartment (AC, GC).</p>b<p>Cultures were infected with HSV-1 17syn⁺ in the presence of 50 µg/ml ACV, harvested at 2 dpi and dispersed.</p>c<p>Genome levels per dispersed TGE were quantified by qPCR (mean of three experiments).</p>d,e<p>HSV-1-positive cells and neurons per dispersed TGE were detected and quantified by immunofluorescence with a HSV-1-specific rabbit antiserum (d), a monoclonal antibody against the 200 kD neurofilament marker (e) and DAPI (d,e) (mean of three experiments).