EGF signaling from the germline to the soma decreases the frequency of GSC divisions.

<p>(A–C) The percentage of pHH3-positive GSCs for each indicated genotype. (A) The expression of <i>s-spi</i> in germ cells rescues the hyper-proliferation of GSCs in <i>spi^77-20</i> testes. (B) GSCs in <i>stet¹/stet³</i> testes showed an increased M-phase index compared to <i>w¹¹¹⁸</i> testes. (C) RNAi-mediated knock-down of EGFR in the soma causes a higher GSC M-phase index, ***p-value≤0.0001.