<i>Cyp301a1</i> is a conserved insect P450.

<p>(<b>A</b>) Phylogenetic analysis of Cyp301A1 and Cyp301B1 in the insect species. Values shown represent 1000 bootstrapping values. <i>D. melanogaster</i> (<i>D.mel</i>) Cyp49A1 was used as an outgroup. <i>D.ere</i> = <i>D. erecta, D.sec</i> = <i>D. sechellia</i>, <i>D.sim = D. simulans</i>, <i>D.yak</i> = <i>D. yakuba</i>, <i>D.ana</i> = <i>D. ananassae</i>, <i>D.per</i> = <i>D. persimilis</i>, <i>D.pse</i> = <i>D. pseudoobscura</i>, <i>D.gri</i> = <i>D. grimshawi</i>, <i>D.vir</i> = <i>D. virilis</i>, <i>D.moj</i> = <i>D. mojavensis</i>, <i>D.wil</i> = <i>D. willistoni</i>, <i>A.gam</i> = <i>A. gambiae</i>, <i>N.vit</i> = <i>Nasonia vitripennis</i>, <i>A.mel</i> = <i>Apis mellifera T.cas</i> = <i>Tribolium castaneum</i>, <i>B</i>.<i>mor</i> = <i>Bombyx mori</i>, <i>P.hum</i> = <i>Pediculus humanis</i>. (<b>B</b>) Alignment of the heme-binding domain of Cyp301A1 and Cyp301B1 from representative insect species. Conserved residues in the heme-binding domain consensus sequence (PxxxGxxxCxG) are highlighted in blue. The conserved Phenylalanine (F) in the second position (highlighted in green) in Cyp301B1 orthologs is changed to a Tyr (Y) (highlighted in red) in all Cyp301A1 orthologs.