Vpr significantly enhances replication-competent HIV-1_NLAD8 infection in MDDCs.

<p>(A) The HIV-1_NLAD8 and HIV-1_NLAD8ΔVpr virus stocks produced from HEK293T cells were analyzed by immunoblotting for the presence of Vpr. (B) PHA-activated PBMCs and (C) MDDCs were infected with 5 ng and 20 ng of p24, respectively, from HIV-1_NLAD8 and HIV-1_NLAD8ΔVpr virus and levels of p24 capsid released into the media during virus replication were assayed over a period of 10 days and 7 days post infection for PBMCs and MDDCs, respectively. The data shown represents one of three independent experiments carried out with three individual donors, and error bars represent standard deviation of triplicate infections. Statistically significant differences are indicated by the asterisks (<i>P<</i>0.05).