Peripheral deletion of Ova-specific CD8⁺ can be evidenced in double transgenic [OT-I×SM-Ova]F1 mice after thymectomy.

<p>(<b>A</b>) Double transgenic [OT-I×SM-Ova]F1 and control OT-I mice were thymectomized at day 0. The total numbers of Vα2⁺Vβ5⁺CD8⁺ cells were determined from blood samples one day before thymectomy and 60 or 90 days after thymectomy. (<b>B</b>) Representative flow cytometry overlay profile illustrating the level of CD44 expression in peripheral blood cells from unmanipulated [OT-I×SM-Ova]F1 or OT-I control mice in the gated Vα2⁺Vβ5⁺CD8⁺ population. (<b>C</b>) Representative flow cytometry profiles and a scatter plot graph illustrating the percentage of CD44^highCD62L^high cells found in blood samples from unmanipulated [OT-I×SM-Ova]F1 or OT-I control mice in the gated Vα2⁺Vβ5⁺CD8⁺ population. Data are representative of 2 independent experiments with 8–9 mice per group.