Effect of JNK silencing on downstream substrates of insulin signaling.

<p>Cells were transfected with <i>Jnks</i> (si1, si2, si3) or GFP (siGFP) siRNAs for 2 days and then exposed to cytokines (4 hrs). Western blot analysis was performed to determine (<b>A</b>) phospho-GSK3β or (<b>B</b>) phospho-FoxO3A and FoxO3A. Equal protein loading was assessed by blotting membranes with an antibody against tubulin. (<b>C</b>) Graphical presentations summarizing the effects of the different <i>Jnk</i> siRNAs <i>vs</i> siGFP in cytokine-treated cells; control values are set to 1. The data are the means±SD of three independent experiments. (**P<0.01) for cyto-Mix-siGFP <i>vs</i> cyto-Mix-si1, and cyto-Mix-si2. (***p<0.001) for cyto-Mix-siGFP <i>vs</i> cyto-Mix-si3. Significant differences were obtained for phospho-GSK3β in si1, si2, and si3 <i>vs</i> siGFP (***p<0.001).