Inhibitory activities of native guinea-pig serum, heat-inactivated serum, and RDE-treated+heat-inactivated serum against reverse genetic viruses.

<p>Guinea pig sera were incubated with rgPR8 viruses that contain HPAI H5N1 HANA, HA alone or NA alone. The viral inhibitory activities of the test sera were determined in MDCK cells inoculated with the serum-virus mixtures by ELISA. The results are shown as mean of the percentages of viral inhibition derived from quadruplicate experiments. Error bars represent the standard deviation. * There are statistically significant differences between the inhibitory activity of the native serum and the heat-inactivated serum or the native serum and the RDE-treated+heat-inactivated serum (<i>p</i>≤0.05). ** There are statistically significant differences in the serum inhibitory activity as compared between the reassorted PR8 virus and the rgPR8-H5 HA virus or the reassorted PR8 virus and the rgPR8-H5 HANA (<i>p</i>≤0.05).