Effects of mifepristone on the phosphorylation level of MAPK in uNK cells.

<p>A, a representative immunoblot is shown after the treatment of uNK cells with mifepristone (1.0 µM) for 0, 15, 30, 60, and 120 min. Uterine NK cells were isolated and cultured in the medium without IL-2 for 12 h as a negative control of MAPK/ERK activation. In the drug treatment group, uNK cells were cultured in the medium supplemented with IL-2. Uterine NK cells were cultured in the medium with IL-2 and IL-15 for 15 min as a positive control. Immunodetection of MAPK members used specific antibodies for phosphorylated and total proteins of ERK1/2, P38 and JNK. B, densitometric scans of triplicate blots are shown. Experiments were independently repeated 3 times in each group. Data were analyzed using ANOVA and expressed as means ± SEM. *, <i>P</i><0.05.