Effects of mifepristone on the ERK signaling pathway in uNK cells.

<p>Western blot of p-ERK and total ERK in uNK cells treated with mifepristone ± cortisol. To detect p-ERK and total ERK, total cell lysates were harvested after a 30 min treatment with mifepristone (1.0 µM) ± cortisol (1.0 µM). Uterine NK cells were isolated and cultured in the medium without IL-2 for 12 h as a negative control of MAPK/ERK activation. In the drug treatment groups, uNK cells were cultured in the medium supplemented with IL-2. Uterine NK cells were cultured in the medium with IL-2 and IL-15 for 15 min as a positive control. A typical blot (A) and densitometric scans of triplicate blots (B) are shown. Data were analyzed using ANOVA and expressed as means ± SEM. *, <i>P</i><0.05.