Infectivity of HIV-1 particles decreases upon treatment with MEK inhibitor.

<p>293 T cells were transfected with 5 µg pNL4-3 and 0.5 µg 3x-Flag-PAP or pcDNA3 plasmids. Cells transfected with 3x-Flag-PAP plasmid or untransfected were also treated with PD98059 (20 µM). Virus particles were purified from the media of cells collected 40 hours following transfection and equivalent amounts of virus particles (3 ng) were used to infect 1×10⁵ 1G5 cells. Cells were washed and re-fed with fresh medium containing AZT (10 µM) 8 hours following infection and incubated for an additional 40 hours. Cells were harvested, lysed and a luciferase assay was performed to determine relative virus infectivity. Values are means ± S.E. from triplicate samples of three different experiments.