Aspirin-induced alterations in the GSH pool.

<p>HepG2 cells were treated with 5 µmol/ml (<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0036325#pone-0036325-g001" target="_blank">Figure 1A</a>) and 10 µmol/ml (<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0036325#pone-0036325-g001" target="_blank">Figure 1B</a>) of ASA for 48 h and GSH levels in the mitochondria and post mitochondrial supernatant (PMS) were measured by enzymatic method as described in the <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0036325#s2" target="_blank">Materials and Methods</a>. Results are expressed as mean ± SEM of three determinations. Figure also represents measurement of GSH levels after treatment of cells with 100 µM BSO for 18 h alone or after treatment with 10 mM NAC for 2 h prior to the ASA treatment. The values are expressed as mean ± SEM of three determinations. Asterisks (*) indicate significant difference (P≤0.05) from control values, # indicate significant difference (P≤0.05) from ASA treated group and δ indicate significant difference (P≤0.05) as compared to BSO+ASA treated group.