Effects on mucin concentration and host cell adhesion on viability and cytokine production after infection.

<p>A) <i>H. pylori</i> J99 wt proliferation with patient 1 soluble tumor mucins and 50 µg/mL glycoconjugates after 24 h culture in RPMI 10% FBS, prior to MKN7 infection, expressed as fold change from start of culture. B) Binding of <i>H. pylori</i> to MKN7 cells after 24 h infection expressed as percentages less binding than untreated <i>H. pylori</i>. C) Viability of MKN7 cells measured by cell metabolic activity (WST-1 assay) after 24 h infection. Data are shown after subtracting the small signal produced by the presence of bacteria in the assay. D) Percentage of live, early and late apoptotic cells after 24 h infection measured by flow cytometry (Annexin V/7AAD). E) Concentration of IL-6 and IL-8 in cell culture media after 24 h infection (One out of two experiments carried out at different concentrations with similar results are shown). (***p≤0.001, **p<0.01, *p<0.05, ANOVA, Dunnett t-test, compared to J99 without mucins or with HSA control. Independent Student's t-test was used to compare between Le^b and sialyl-Le^x glycoconjugates.)