Methylation analysis of the ERα promoter by BSP in breast cancer cells.

<p>(A)Shown is a representative bisulfite sequence analysis for the high CpG region of the ERα promoter, which contains 28 CpG dinucleotides. For MDA-MB-231 and MCF-7 cell lines (10 replicates each), methylated CpGs are designated by closed circles; unmethylated CpGs are designated by open circles. (B)Statistical chart of methylation analysis; the results shown represent the means ± S.E. of three independent experiments. Significant differences from the controls as determined by Student's t test are indicated by asterisks (<i>P</i> <0.05).