Low correlation between 14-3-3 protein levels and LDH activity in CSF of CJD patients.

<p>CSF samples from CJD patients (N = 40) and non-CJD controls (N = 16), were obtained by lumbar puncture. Then, <b>A.</b> 14-3-3 levels in CSF were analyzed by Western blot. <b>B.</b> Relative levels of 14-3-3 protein band were quantified using densitometric analysis. <b>C.</b> LDH activity was measured in each sample as described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0036159#s4" target="_blank">Material and Methods</a>. <b>D.</b> A correlation between 14-3-3 protein and LDH activity from panels B and C is presented. <b>E.</b> Quantitative analysis of total protein levels in CSF samples of panel A was performed. In B, C and E the red line represents the mean of all samples analyzed. Statistical significance was calculated using Student's t-test.