Altered PrP expression pattern in CSF of CJD patients.

<p>CSF samples from CJD patients (N = 40) and non-CJD controls (N = 16), were obtained by lumbar puncture. <b>A.</b> PrP levels in CSF were examined by Western blot. <b>B.</b> and <b>C.</b> Quantification of relative levels of total PrP protein in CSF and the ratio of the PrP band 1 (upper) and PrP band 2 (lower) in CSF, respectively. <b>D.</b> Comparison between ratios of PrP band 1 and 2 in sCJD versus fCJD <b>E.</b> Correlation of total PrP protein levels with 14-3-3 protein in each CSF samples presented in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0036159#pone-0036159-g001" target="_blank">Fig 1</a>. In B, C and D the red line represents the mean of all samples analyzed. Statistical significance was calculated using Student's t-test.