Alterations in PrP expression pattern in CSF are specific to CJD patients.

<p>CSF samples from healthy control subjects (N = 3), CJD patients (N = 5), dementia patients (N = 3) and paraparesis patients (N = 3), were obtained by lumbar puncture. <b>A.</b> Assessment of PrP levels (two upper panels with different exposure), and 14-3-3 protein levels (third panel) by Western blot analysis, and total protein content by Ponceau Red staining (lower panel, a selected band is presented). <b>B.</b> and <b>C.</b> Quantification of relative total PrP protein levels in CSF and the ratio of the PrP band 1 (upper) and PrP band 2 (lower) in CSF, respectively. The asterisk in A indicates one sample with a technical problem in the blot detection that was excluded for quantification of panel B and C. In B and C the red line represents the mean of all samples analyzed. Statistical significance was calculated using Student's t-test.