Effect of IFN-λ3 on the key elements in JAK-STAT pathway in uninfected macrophages.

<p>Seven-day-cultured macrophages were treated with or without IFN-λ3 (100 ng/ml) for 24 h. Total nucleotide were extracted from macrophages and subjected to the real-time reverse transcription polymerase chain reaction (RT-PCR) assay for mRNA expression of TLRs (<b>A</b>), IRFs (<b>B</b>) and the key elements in IFN-λ3 signaling pathway (<b>C</b>). The data shown were the mean ± standard deviation of triplicate culture (<i>*P<0.05, **P<0.01</i>).