Mib1-null DCs fail to activate Notch signaling.

<p>A, LPS-stimulated <i>Mib1^f/f</i> or <i>Mx1-Cre;Mib1^f/f</i> DCs were cultured with Notch1-expressing C2C12 cells transfected with WT or MT CBF luciferase constructs, and 24 h after the coculture, luciferase activity was measured. Results are representative of two independent experiments. <i>B</i>, Western blot analysis of Notch1 ICD (NICD) in negatively purified OT-II CD4⁺ T cells before (<i>None</i>) and 6 h after coculture with peptide-pretreated <i>Mib1^f/f</i> or <i>Mx1-Cre;Mib1^f/f</i> DCs. <i>C</i>, Quantitative real-time RT-PCR analysis of Notch target genes, Hes1 and Hes5, in CD4⁺ T cells before (<i>None</i>) and after coculture with <i>Mib1^f/f</i> or <i>Mx1-Cre;Mib1^f/f</i> DCs. Results are representative of three independent experiments. Data represent mean ± SD; *, P<0.05; **, P<0.001.