Real-time RT-PCR analysis of Ss-Sl2 transcript in different sclerotial developmental stages of <i>S. sclerotiorum</i>.

<p>S0 (early) = the early stage of vegetative growth (cultured on PDA plates for 1 day); S0 (later) = the later stage of vegetative growth (cultured for 3 days); S1 = the initiation stage of sclerotial development (cultured for 4 days); S2 = condensation stage (cultured for 5 days); S4 = consolidation stage (cultured for 6 days); S6 = maturation stage (cultured for 7 days). The expression level of <i>Ss-Sl2</i> cDNA measured by RT-PCR was normalized to that of <i>actin</i> cDNA in extracts from each developmental stage. The abundance of cDNA from S0 (early) samples was assigned a value of 1. Bars indicate standard error.