Histopathological examination of MSCs and short-term cyclosporine A (CsA) decreases allotransplant rejection.

<p>Histopathological evaluation revealed signs of severe rejection (grade III) including diffuse, dense polymorphic inflammatory cells infiltrate beneath the dermal-epidermal junction in grafted skin and lymphoid gland tissues of untreated controls at 2 weeks post-transplantation (arrow). Animals in the MSC alone group showed moderate rejection (grade II) with a slighted dense dermal infiltrate inflammatory cells around dermal blood vessels and diffusing interstitially in lymphoid gland tissues at 2 weeks post-transplantation. Animals in the CsA group showed a dense perivascular dermal lymphocytic infiltrate, extending focally into the epidermis (grade II–III) at 6 weeks post-transplantation. Animals in the MSC-CsA group revealed small number perivascular lymphocytes infiltrated in the alloskin (grade I) or lymphoid gland tissue (grade I) when compared to other groups at 6 weeks post-transplantation. Photo magnification is 100× in skin and lymphoid gland tissue. Scale bar = 10 µm.