Effect of arginase inhibitor norNOHA on the NO production and <i>T. gondii</i> growth in BALB/c mouse peritoneal macrophages.

<p>(A) Arginase activity in mouse peritoneal macrophages treated with LPS (0.5 µg/ml) only or LPS (0.5 µg/ml)+norNOHA (500 µM) for 24 hrs, measured by a colorimetric assay; enzyme activity is the output of urea secreted from lysed macrophages. (B) NO production measured by the Griess reaction in mouse macrophages treated with LPS (0.5 µg/ml) only or LPS (0.5 µg/ml)+norNOHA (500 µM) for 24 hrs. (C) Number of <i>T. gondii</i> per 100 macrophages counted at 24 hrs after infection in LPS (0.5 µg/ml) only or LPS (0.5 µg/ml)+norNOHA (500 µM) treated mouse macrophages. Mean ± SEM and significant differences (*<i>p</i><0.05, ** <i>p</i><0.01).