Lamina propria DCs, but not splenic DCs, express high levels of TLR5 protein.

<p>(<b>a</b>) Quantitative real-time PCR of <i>Tlr5</i> expression in the spleen, liver, thymus and intestine homogenates of C57BL/6 mice normalized to <i>18S</i> mRNA expression. **<i>P</i><0.01; compared to spleen. (<b>b</b>) Quantitative real-time PCR of <i>Tlr5</i> expression in different intestinal fragments, including duodenum, jejunum, ileum, cecum, proximal colon (P. colon) and distal colon (D. colon), as compared to spleen <i>Tlr5</i> expression. (<b>c</b>) TLR5 expression on splenic DCs (Spl-DC), small intestinal DCs (SI-DC) and large intestinal DCs (LI-DC) from pooled three mice was determined by flow cytometry. Plot numbers represent the percentage of CD11c⁺ cells in the respective quadrants. (<b>d</b>) Aggregate data of percentages of TLR5⁺ cells among CD11c⁺ cells. *<i>P</i><0.05; **<i>P</i><0.01; compared to splenic DCs. Data are aggregate (a, b, d; mean ± SEM) or representative (c) of three experiments, 2–3 mice for each experiment.