Tumorigenic and invasive potential of cells from each fraction.

<p>(A) Column chart showing the tumor incidence in each group following the injection of 1×10⁵ cells. (B) Column chart showing the tumor incidence in each group following the injection of 1×10⁴ cells. (C) Subcutaneous tumors in mice following the implantation of 1×10⁴ cells from each group. (D) Representative tumors were selected accordingly. (E) Histological analysis of subcutaneous tumors by H&E staining, showing HCC-specific features (irregularly packed tumor cells, hyperchromatic nuclei, high density of microvessels and pleomorphism). (F) The livers of the mice were damaged to different extents by injecting 1×10⁴ cells from each group (black arrows). (G) H&E staining of liver lesions. Original magnification: 200× (E, G).