Flow cytometry analysis of the intracellular expression of IFN-gamma, IL-4 and IL-17 at CD4⁺ T cells after coculturing.

<p>(A) CD4⁺ T cells from different coculturing groups were stained with specific antibodies to IFN-gamma, IL-4 and IL-17 as described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0032060#s2" target="_blank">materials and methods</a>. The histograms shown are from a representative results of six independent experiments are shown. (B) The mean relative fluorescence intensity of intracellular cytokine was quantified after ITGB4 silencing.