Morphology of rat nestin-positive cells and structures in invasive and angiogenic GBM biopsy xenografts.

<p>Elongated, bipolar rat nestin-positive cells (green, arrowheads) accumulate at the infiltrative edge of a biopsy xenograft (red) (<b>A</b>). Magnified view of cells in the tumor invasive zone with infiltrative single glioma cells [(arrowheads, rat nestin-positive cells (green); arrows, human nestin-positive glioma cells (red)] (<b>B</b>, <b>C</b>). A rat nestin-positive host cell (green) closely associated with an invasive human nestin-positive tumor cell (red, arrow), with the two elongated cell bodies juxtaposed to each other (arrowheads) (<b>C</b>). Rat nestin-positive vascular elements (green) in a high-generation human GBM xenograft (red) that induces angiogenesis (<b>D</b>). Host nestin-positive glomerular microvascular proliferation (green) typical of human GBM pathology recapitulated in the rat brain (<b>E</b>). Elongated rat nestin-expressing cells with terminal processes and star-shaped cells (green, arrowheads) in a GBM xenograft with locally invasive growth (red) and induction of angiogenesis (arrow) (<b>F</b>). Cells with astrocytic morphology stained for rat nestin (green, arrowheads) in a low-generation highly infiltrative xenograft with low tumor cell density (red) <b>G</b>). <i>Bars </i><b>B</b>, <b>C</b> 10 µm, <b>F</b> 50 µm, <b>A</b>, <b>D</b>, <b>E</b>, <b>G</b>, <b>H</b> 100 µm.