Apoptosis assessment of B16-F10 melanoma cells submitted to a heat treatment (HT), 45°C for 30 min.

<p>Flow cytometry analysis of Annexin-FITC/PI uptake to evaluate the percentage of cells in early and late apoptosis. Following the heat treatment, cultures were maintained at 37°C for 2 weeks. A: control cultures (kept at 37°C during the culture time); B: heat-treated cultures; C: detail of the heat-treated cultures during the first 24 h after heat exposure. *Significantly different from control (cultures kept at 37°C).