Six-week-old neurons formed after a pSE insult show decreased scaffolding protein expression but unaltered expression of adhesion molecules important at excitatory synapses.

<p>Representative photomicrographs of PSD-95 (<i>A</i>), NL-1 clusters (<i>C</i>), and N-cadherin (<i>F</i>), on GFP+ dendritic spines (arrow heads) and shafts (arrows). Note the distribution of NL-1 clusters mainly on the dendritic shaft. <i>B</i>, Cluster density of PSD-95 expression on dendritic spines (iML and oML pooled). <i>D</i>, Image showing co-localization of NL-1 clusters with VGLUT1 (arrow) in ML confirming the presence of NL-1 at excitatory synapses. A few clusters did not co-localize (arrow head). <i>E</i>, Images showing VGLUT1 located on a spine (arrow head) and on the dendritic shaft (arrow) of GFP+ dendrites. Means ± SEM, n = 5 control and n = 4 pSE group *, P<0.05 unpaired t-test compared to control group. Scale bars are 1 µm (in <i>A</i> for <i>A</i>, <i>C</i>, <i>F</i> and in <i>D</i> and in <i>E</i>).