ROS dependent IL-12 production from LAB stimulated macrophage.

<p>(A) Peritoneal macrophages were cultured with 1 µg/ml KW3110, ATCC53103 or 1 µM CpG for 24 hrs in the presence of 0, 0.1, 0.5 or 2 mM apocynin (Apo) or 0, 0.01, 0.05 or 0.2 mM propyl gallate (PG). IL-12p40 and IL-12p70 in the cell culture supernatant were measured by ELISA. (C) Peritoneal macrophages were cultured with FITC-labeled identical KW3110 (3 µg/ml) in the presence of Apocynin or propyl gallate. Phagocytosis index was calculated in the same manner as described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0035880#pone-0035880-g001" target="_blank">Fig. 1A</a>. Data are representative of three independent experiments.