Quantification of angiogenic cytokines in conditioned media.

<p>(A) Conditioned GM and (B) original unconditioned GM demonstrate serum contains trace amounts of angiogenic cytokines save the positive (PC) and internal controls (IC). (C) Stacked column chart of fluorescence (×1000) of predominant angiogenic cytokines and (D) VEGF fluorescence as a function of conditioned media. Data is presented as median subtracted background fluorescence intensity and normalized to positive controls and excluding inherent serum levels for pooled conditioned media for n = 4.