PKC phosphorylation is not directly regulated by ANX7.

<p><b>A.</b> S2-013 and PANC-1 cells were prelabeled with [³²P]-orthophosphoric acid and stimulated or not with PMA. ANX7 was immunoprecipitated, separated by SDS-PAGE, and analyzed by autoradiography. An immunoblot was performed on the cell lysate as a loading control. <b>B. </b><i>In vitro</i> phosphorylation assays to investigate the effect of BART and ANX7 on PKC-phosphorylation. Purified rat brain was incubated with recombinant ANX7 with or without recombinant BART. The reaction products were analyzed by an ELISA assay. ABS on <i>Y</i>-axis means absorbance at 492 nm as reference measured with a microplate reader.