PKCα is associated with increased cell invasion by knockdown of BART and ANX7.

<p><b>A.</b> BART RNAi S2-013 cells and S2-013 cells transiently transfected with ANX7-siRNA were pretreated with or without Ro-32-0432. The activity of PKCα and η was assessed by Western blotting. <b>B.</b> Cells shown in (A) were pretreated with or without safingol. The activity of PKCα and PKCη was assessed by Western blotting. <b>C.</b> The effect of Ro-32-0432 on cell invasion was investigated using the transwell invasion assay. Migrated cells in four fields per group were counted. Data are representative of three independent experiments. <i>Columns</i>, mean; <i>bars</i>, SD. *<i>p</i><0.001 compared with non-treated cells. <b>D.</b> The effect of safingol on cell invasion was investigated using the transwell invasion assay. Data are representative of three independent experiments. <i>Columns</i>, mean; <i>bars</i>, SD. *<i>p</i><0.001; **<i>p</i><0.003 compared with non-treated cells. <b>E.</b> The effect of rottlerin and pseudosubstrate PKCη inhibitor on cell invasion was investigated using the transwell invasion assay. Data are representative of three independent experiments. <i>Columns</i>, mean; <i>bars</i>, SD.