Effect of 9-CRA, ATRA, and androgen on junctional transfer in LNCaP-32 cells.

<p>LNCaP-32 cells, seeded in 6 cm dishes, were grown to 70% confluence. Junctional transfer was measured after microinjecting fluorescent tracers as described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0032846#s2" target="_blank">Materials and Methods</a>.</p>a<p>: The number of fluorescent cell neighbors (mean ± SE) 1 min (Lucifer Yellow), 3 min (Alexa-488) and 15 min ( Alexa-594) after microinjection into test cell. The total number of injection trials is shown in parentheses.</p>b<p>: Cells were treated for 48 h with 9-CRA and ATRA (1 µM) and MB (2.5 nM).