Serum IgG subclass responses in goats subcutaneously vaccinated with inactivated CpHV-1 plus MF59™.

<p>Vaccines were given on day 0 and day 10. Sera were collected on day 20 to determine CpHV-1-specific IgG1 titers (white histograms) and CpHV-1-specific IgG2 titers (black histograms) by ELISA. Data are expressed as arithmetic mean ± SD. Data reported are cumulative from 4 independent experiments performed as described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0034913#s2" target="_blank">Materials and Methods</a> with a total of 8 goats in the naïve group, a total of 8 goats in the inactivated CpHV-1 only vaccinated group and a total of 12 goats in the inactivated CpHV-1 plus MF59™ vaccinated group. A single asterisk (*) denotes probability, p, <0.05 that the serum IgG1 titers are equivalent to the serum IgG2 titers in goats vaccinated with inactivated CpHV-1 plus MF59™. The Students' <i>t</i>-test was employed to study statistical differences.