Repression of the human resistin promoter by Sirt1.

<p><b>A</b>, Gene reporter assays using 3.0 kb of the human resistin promoter in 293 T cells transfected with c-Jun, Sirt1 or empty control plasmids and treated for 24 h with resveratrol (20 µM) or vehicle before quantification of the luminescent signal. Bars are representative of 3 experiments done in triplicate. * indicates a significant difference between groups. <b>B-C</b>, ChIP assay of the AP-1 response element on the resistin promoter using antibodies against Sirt1, IgG, or c-jun on U-937 cells treated with 1 µg/ml LPS or vehicle for 24 h.