Isotype of anti-BmHsp12.6 IgG antibodies in the sera of mice.

<p>Mice were immunized with <b>A</b>) BmHsp12.6, <b>B</b>) BmHsp12.6αc or <b>C</b>) N-BmHsp12.6 using homologous DNA vaccine regimen or a heterologous prime boost approach. Control mice were immunized with vector alone or adjuvant. Isotype specific ELISA was performed as described in the <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0034077#s2" target="_blank">methods</a> section. Bars represent mean O.D ± SD from five mice per group. * Significant (p<0.005) compared to all the other groups.