Expression of PTN in the chicken oviduct.

<p>[A] Results of RT-PCR analysis using cDNA templates from each segment of the chicken oviduct with chicken <i>PTN</i> and chicken <i>ACTB</i>-specific primers. [B] <i>In situ</i> hybridization analyses of <i>PTN</i> mRNAs in the chicken oviduct. Cross-sections of the infundibulum, magnum, isthmus and shell gland of the chicken oviduct were hybridized with antisense or sense chicken <i>PTN</i> cRNA probes. [C] Immunoreactive PTN protein in the chicken oviduct. For the IgG control, normal rabbit IgG was substituted for the primary antibody. Sections were not counterstained. Legend: LE, luminal epithelium; GE, glandular epithelium. <i>Scale bar</i> represents 100 µm. <i>See </i><a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0034215#s4" target="_blank"><i>Materials and Methods</i></a><i> for complete description</i>.