Bisulfite sequencing of CpG sites in the upstream region of the <i>PTN</i> gene.

<p>[A] Schematic of the four CpG sites in the promoter region of the PTN gene are indicated by the heavy black vertical lines. The numbers on the line indicate positions relative to the transcription start site. Legend: a, complex contains E2F and p107 (E2F+p107); b, upstream stimulatory factor (USF also known as gamma-factor). [B] The CpG methylation status in the upstream region of the <i>PTN</i> gene was analyzed in normal and ovarian cancer cells from hens by bisulfate sequencing. Each circle indicates a CpG site in the primary sequence, and each line of circles represents analysis of a single cloned allele. Closed and open circles are methylated and unmethylated CpGs, respectively. [C] Comparison of the sequences around CpG regions of <i>PTN</i> genes of chicken, mouse, and human. The 5′ flanking region (about 2.2 kb) of mouse and human <i>PTN</i> was compared to that of chicken <i>PTN</i> to identify differences in sequences around each CpG sites among those species.