Correlations between key components of the canonical, non-canonical and atypical NFκB signalling pathways.

<p>Protein levels of activated NFκB were examined in each sample by immunoblotting as previously described. Levels of both nuclear p65 and nuclear phospho-p65 were shown to correlate highly with nuclear levels with Rel-B (A and B, R² = 0.8145 and R² = 0.6288 respectively). No correlation was detected between nuclear levels of p65 and p50 (C, R² = 0.06856), nuclear Rel-B and p52 (D, R² = 0.00008) or nuclear p65 and IκBα (E, R² = 0.0077). Collectively these results suggest that neither the canonical, non-canonical nor the atypical signalling pathways are responsible for the observed differences in NFκB activation.