Treatment of U937 macrophages with MA ± LPS resulted in increased translocation of the p50 subunit of NF-κB.

<p>1×10⁶ U937 monocytes were differentiated into macrophages with 100 nM PMA for 48 h. Cells were treated with MA at 0, 24 and 48 h then stimulated with 100 ng/ml LPS for 3 h. Cytosolic and nuclear protein fractions were purified by electrophoresis on 10% SDS gel and transfer to a PVDF membrane. The cytosolic fractions and nuclear fractions are shown in a representative western blot. The expression of the p50 subunit was normalized to the appropriate compartmental housekeeping genes (Lamin B for nucleus and GAPDH for cytoplasm). The means and SE values are from three independent experiments.