Analysis of mGluR2/3 and mGluR7 surface vs. intracellular distribution in rat brain synaptosomes.

<p>(A) Rat brain atlas coordinates (adapted from <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0034299#pone.0034299-Paxinos1" target="_blank">[77]</a>) used for tissue dissection and synaptosome preparation. (B) Representative immunoblots showing surface vs. intracellular distribution of mGluR2/3 and mGluR7 receptors (as well as marker proteins: calnexin, ERK1/2 and RasD1/AGS1) in synaptosomal fraction prepared from the dSTR. P2* - synaptosomal fraction resolved under strongly reducing conditions (100 mM DTT); T – total synaptosomal fraction after biotinylation resolved under weakly reducing conditions (>10 mM DTT); NB – non-biotinylated (intracellular) proteins; B – biotinylated (surface) proteins isolated by precipitation with streptavidin-agarose beads.