Presence and expression of the four larval midgut-predominant genes.

<p>(A) Analysis of presence and expression of the four larval midgut-predominant genes (<i>Slmg2</i>, <i>Slmg7</i>, <i>Slmg9</i> and <i>Slmg17</i>) in <i>Spodoptera litura</i> by using genomic DNA and cDNA; (B) PCR amplification of the four genes in <i>S. litura</i>, <i>C. fumiferana</i>, <i>H. armigera</i>, <i>B. mori</i> and <i>D. melanogastera</i> using genomic DNA as templates. (C) Northern blot analysis of expression of the homologous genes in <i>S. litura</i>, <i>C. fumiferana</i>, <i>H. armigera</i>, <i>B. mori</i> and <i>D. melanogastera</i>. Ten micrograms of total RNA isolated from the midgut (MG) and the whole body (WB) was separated on denaturing 1% MOPS-formaldehyde gel. ³²P-labeled EST fragments of corresponding <i>S. litura</i> genes were used as probes. Ethidium bromide stained rRNA indicates the equal loading of total RNA. <i>Spli</i>-221: an embryogenic cell line of <i>S. litura</i>; L5: 5^th instar larvae; L6D0: the day (white head) of ecdysis from 5^th to 6^th instar stage; L6D3: 3 day after ecdysis into 6^th instar stage; P: pupal stage; A: adult stage.