Effect of elevated glycerol levels on hypertonic stress-induced aggregation of Q35::YFP.

<p><i>A:</i> Aggregation of Q35::YFP measured after a 24 h exposure of <i>osm-11</i> or acclimated worms to media containing 200–600 mM NaCl. (<i>n</i> = 13–15). *P<0.01 compared to acclimated animals. <i>B:</i> Time course of motility changes in <i>osm-11</i> and acclimated worms exposed to 600 mM NaCl. Acclimated worms become paralyzed within 10–15 min due to water loss. <i>osm-11</i> mutants lose less water due to elevated glycerol levels (see <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0034153#pone-0034153-g001" target="_blank">Figure 1A</a>) and therefore remain motile compared to acclimated worms. (<i>n</i> = 13–21). *P<0.0001 compared to acclimated animals.