SapC -DOPS-CVM targets primarily CD11b+Gr-1+ joint cells in the CIA model of arthritis.

<p>Cells were stained with antibodies to cell surface markers as indicated. FACS plots are representative from one animal, with bar graphs indicating the means +/− the SEM of groups. (A) Dot plots indicate cell populations from mice treated as indicated that are CVM+ and positive for other cell surface markers as indicated on left. (B) CVM+ cells. Columns represent mean of total cells that are CVM+ +/− SEM (error bars) with treatment of animals as indicated. * p<0.0001 Mann Whitney. (C) Columns indicate means +/− the SEM (error bars) of the percentage of total cells that are CVM + and positive for the indicated cell surface marker. * = p<0.05 Mann Whitney. (D) Dot plots show forward (FSC) and side scatter (SSC) for joint populations with gating for CVM +cell populations versus side scatter (SSC). Gated CVM + cells are further analyzed for combinations of the specific cell-surface markers CD11b, CD11c and Gr-1 as indicated. (E) Columns represent the mean +/− the SEM (error bars) of the percentage of total cells that are positive for CVM, CD11b, and also positive for either CD11c or Gr-1. N = 4, No CII, SapC-DOPS-CVM; 8, CII+, SapC-DOPS-CVM+ * p<0.05, Mann Whitney.