MLN4924 sensitizes breast cancer cells to radiation.

<p>(<b>A&B</b>) Cullin deneddylation: Subconfluent breast cancer cells were treated with MLN4924 at indicated concentrations or for indicated time periods, followed by immunoblotting (IB) with β-actin as a loading control. (<b>C</b>) Radiosensitization by MLN4924: SK-BR-3 or MCF7 cells were seeded in 60-mm dishes in duplicate and treated with MLN4924 (30 nM) and radiation at various doses. The colonies with more than 50 cells were counted after 7–11 days. Surviving fraction was calculated as the proportion of seeded cells following irradiation to form colonies relative to that of untreated cells (mean ± SEM) (n = 3 for SK-BR-3 cells and n = 2 for MCF7 cells). SER was calculated as the ratio of the mean inactivation dose under untreated control conditions divided by the mean inactivation dose after MLN4924 treatment.