MLN4924 alters cell cycle profile and induces apoptosis.

<p>SK-BR-3 cells were treated with MLN4924 at 100 nM for 24 hrs or 48 hrs (<b>A&B</b>), alone or in combination with radiation (6 Gy), followed by FACS analysis for cell cycle profile (<b>A</b>), sub-G1 population for apoptosis (<b>B</b>), DNA fragmentation (48 hrs treatment) (<b>C</b>) and Western blotting with indicated antibodies (<b>D</b>). Shown is mean (<b>A</b>) or mean ± SEM (<b>B</b>), (n = 3): **, <i>p</i><0.01. One representative result is shown.